Affinity Capillary Electrophoresis
نویسندگان
چکیده
منابع مشابه
Application of retention factors in affinity electrokinetic chromatography and capillary electrophoresis.
Affinity capillary electrophoresis has become an established approach for performing interaction studies. In affinity electrokinetic chromatography the retention factor, as in liquid chromatography, is useful for describing the migration behavior of the analytes, and is instrumental for assessing the affinity of an analyte for the pseudo-stationary phase. Erroneous use of the retention factor c...
متن کاملKinetic CE: foundation for homogeneous kinetic affinity methods.
Kinetic capillary electrophoresis (KCE) is defined as capillary electrophoresis of species that interact during electrophoresis. KCE can serve as a conceptual platform for development of homogeneous kinetic affinity methods for affinity measurements (measurements of binding parameters and quantitative measurements) and affinity purification (purification of known molecules and search of unknown...
متن کاملDetection of Single-base Mutation by Affinity Capillary Electrophoresis in a Pdms-glass Hybrid Microdevice
An extremely rapid on-chip separation of DNA from its single-base substituted mutant is described. By using affinity capillary electrophoresis (ACE) in a poly(dimethylsiloxane) (PDMS)-glass hybrid microdevice, we achieved the separation within 5 s.
متن کاملHigh-throughput screening of holoprotein conformational stability by dynamic ligand exchange-affinity capillary electrophoresis.
Dynamic ligand exchange-affinity capillary electrophoresis (DLE-ACE) is introduced as a convenient platform for assessing the conformational stability and relative affinity of a holoprotein to different ligands without off-line sample pretreatment, since ligand exchange and protein unfolding processes are integrated in-capillary during electromigration.
متن کاملNoncooled capillary inlet: a source of systematic errors in capillary-electrophoresis-based affinity analyses.
Capillary electrophoresis (CE) serves as a platform for a large family of temperature-sensitive affinity methods. To control the electrolyte temperature, the heat generated during electrophoresis is removed by actively cooling the capillary. Short parts of the capillary, particularly at its inlet, are not actively cooled, however, and the electrolyte in this part is likely to be at an elevated ...
متن کاملRecent advances in affinity capillary electrophoresis (2007).
Papers detailing the use of affinity capillary electrophoresis (ACE) in examining binding parameters between biological species are summarized in this review. The works cited in this review were published during 2007 and were drawn from the major chemical and biochemical journals and especially the major analytical chemistry literature.
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